Anyone who has ever done DNA cloning knows how hard it is to get the sequence you want without accumulating any mutations, especially when amplifying the insert via PCR. Anyone who has ever done DNA sequencing knows how hard it is to get a clean, readable sequence with no bizarre misintepretations by the sequencer's software, or a chromatogram which looks like the frequency plot of a heavy metal track.
I have just looked at the results from sequencing the insert of the vector construct I made. 970 base-pairs of pure, untainted perfection.
Shh, let me enjoy this moment.
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